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114 search results for "digitalMLPA"
  • How can I visualise digitalMLPA reactions using electrophoresis?

    digitalMLPA & Coffalyser digitalMLPA Experimental Setup Sequencing

    There are two methods to visualise digitalMLPA reactions or libraries: using a capillary electrophor...

  • What can I do about impurities in my DNA sample?

    MLPA & Coffalyser.Net Raw Data Interpretation & Troubleshooting

    To reduce the effect of impurities in a DNA sample on a conventional MLPA or digitalMLPA reaction, y...

  • How many samples do I need to include in a digitalMLPA experiment with no dedicated reference samples?

    digitalMLPA & Coffalyser digitalMLPA Experimental Setup Sample Selection & Requirements

    The minimum number of samples required in a digitalMLPA™ experiment without dedicated reference samp...

  • Do mismatches between probes and their target sequences influence (digital)MLPA results?

    digitalMLPA & Coffalyser digitalMLPA digitalMLPA Technique

    Probes can be affected by mismatches between the probe sequence and the sample DNA. The presence of ...

  • Which Illumina reagent kits can be used for digitalMLPA?

    digitalMLPA & Coffalyser digitalMLPA Experimental Setup Sequencing

    Most Illumina Reagent Kits capable of generating 110 nt reads (single-end) are suitable for digitalM...

  • Is the purity and quality of the sample DNA important for (digital)MLPA?

    MLPA & Coffalyser.Net Experimental Setup Sample Selection & Requirements

    Sample purity is very important for conventional MLPA and digitalMLPA while sample quality is usuall...

  • How many digitalMLPA samples can I combine in a single run?

    digitalMLPA & Coffalyser digitalMLPA Experimental Setup Sequencing

    You can calculate the maximum number of digitalMLPA samples that can be included in a single sequenc...

  • Why is digitalMLPA more robust than conventional MLPA?

    digitalMLPA & Coffalyser digitalMLPA digitalMLPA Technique

    While conventional MLPA is already a highly robust technique for the detection of copy number change...

  • Is there a specific DNA extraction method I need to use for (digital)MLPA samples?

    MLPA & Coffalyser.Net Experimental Setup Sample Selection & Requirements

    No specific extraction method is required for conventional MLPA or digitalMLPA, as long as the DNA i...

  • Statement: No Safety Data Sheet Required

    Various

    None of our products contain dangerous substances at concentrations that require the distribution of...

  • What is sample depurination and how do I prevent it?

    MLPA & Coffalyser.Net Experimental Setup Sample Selection & Requirements

    To prevent sample depurination, it is essential that DNA samples contain sufficient buffering capaci...

  • What Library Prep Kit do I select for digitalMLPA in the Illumina Local Run Manager?

    digitalMLPA & Coffalyser digitalMLPA Experimental Setup Sequencing

    When sequencing SALSA® digitalMLPA™ reactions, a run sheet must be prepared in the Local Run Manager...

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