There are two methods to visualise digitalMLPA reactions or libraries: using a capillary electrophoresis device from Applied Biosystems (ABI), or using a non-denaturing system such as the Agilent TapeStation.
Capillary electrophoresis with an ABI device relies on the presence of a FAM dye on one of the primers used in the digitalMLPA reactions. This can be used to visualise relevant digitalMLPA PCR products and detect complete reaction failures. Read more about the experimental setup and the expected peak patterns. SCIEX devices cannot be used for this approach because they are not suitable for the FAM label.
It is also possible to get a broad idea of the formation of digitalMLPA PCR products in reactions or libraries using traditional NGS library assessment methods, such as the Agilent TapeStation, as long as some additional guidelines are followed. Read more about the use of a TapeStation.