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  • MLPA & Coffalyser.Net

MLPA & Coffalyser.Net

MLPA Technique (10)


  • Getting started with MLPA
  • What is MLPA, and how does it work?
  • Video: How does MLPA work?
  • What are the main advantages and limitations of MLPA?
  • How does methylation-specific MLPA (MS-MLPA) work?
View 10 articles

Educational Resources (10)


  • Educational resources for MLPA: e-learning, videos, workshops & webinars
  • Video: How does MLPA work?
  • Video: How to perform an MLPA reaction
  • Video: How to perform SALSA MLPA data analysis for copy number determination in Coffalyser.Net
  • Video: How to analyse SALSA MS-MLPA data in Coffalyser.Net
View 10 articles

Experimental Setup (14)


  • Video: How to perform an MLPA reaction
  • What are the sample and buffer requirements for (digital)MLPA?
  • What control samples should be included in (digital)MLPA experiments?
  • List of verified positive samples that can be used with (digital)MLPA probemixes
  • Is the purity and quality of the sample DNA important for (digital)MLPA?
View 14 articles

Coffalyser.Net Data Analysis Software (55)


  • Using Coffalyser.Net to analyse MLPA data
  • Installation Manual for Coffalyser.Net
  • Reference Manual for Coffalyser.Net
  • Video: How to perform SALSA MLPA data analysis for copy number determination in Coffalyser.Net
  • How do I adjust a bin set (create a manual bin set) in Coffalyser.Net?
View 55 articles

Raw Data Interpretation & Troubleshooting (9)


  • Troubleshooting MLPA results
  • MLPA troubleshooting wizard
  • What are the Q- and D-fragments that are present in SALSA MLPA probemixes?
  • What are the peaks in the no-DNA control of my MLPA experiment?
  • Which factors influence variability of (digital)MLPA probes?
View 9 articles

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