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What do the probe colours in the electropherogram and genomic profile in Coffalyser.Net mean? - Help Centre / MLPA & Coffalyser.Net / Coffalyser.Net Data Analysis Software / Data Analysis / Fragment Analysis - MRC Holland Support

What do the probe colours in the electropherogram and genomic profile in Coffalyser.Net mean?

Coffalyser.Net highlights probe labels in several electropherograms to visualise which probe signals were expected or unexpected, and whether their signals were above or below 10% of the median signal of the reference probes. These highlights are visible in the genomic profile tab of the fragment analysis sample results explorer and in the Electropherograms tab of the comparative analysis sample results explorer, and are intended to help identify unexpected situations at a glance.


Colour Probe signal expected/unexpected Probe signal above/below 10% of the median signal of the reference probes
1. No colour
 
expected above
2. Orange
 
expected below
3. Yellow
 
unexpected above
4. Lavender
 
unexpected below
5. Pale violet red
 
only shown when the median signal of the reference probes is 0 or cannot be calculated

Probes with a lavender label are not counted in the probe counter, but will generate results in the comparative analysis.

The dots that mark individual probe peaks in the Electropherograms tab of the comparative analysis sample results explorer are also coloured based on the results from the comparative analysis. Their colours follow the same rules as the colours of the probe dots in ratio charts (see the Coffalyser.Net Reference Manual, Appendix I).

Note
For information about the colours used in the results of the comparative analysis, see this article.
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The information provided in this material is correct for the majority of our products. For certain applications, the instructions for use may differ. In the event of conflicting information, the relevant instructions for use take precedence.