This article lists the size markers available in Coffalyser.Net™ and their defined fragment sizes. This list may be used to determine if Coffalyser.Net is able to recognise your size marker of choice.
Warning
The Applied Biosystems GeneScan 500 size marker with LIZ or ROX label is the only marker permitted for use with IVD-registered probemixes. See the (MS-)MLPA General Protocol for details. In addition, some size markers are not suitable for the vast majority of MLPA experiments, e.g. because their fragment range is too limited. Use the size marker recommended in the (MS-)MLPA General Protocol for your capillary electrophoresis device if possible.
Frequently used size markers
| Name | Fragments 1 | Examples of Size Standards 2 |
|---|---|---|
| GS500-250 | (35), 50, 75, 100, 139, 150, 160, 200, (250), 300, 340, 350, 400, 450, 490, 500 | Applied Biosystems GeneScan 500; MCLAB Red/Orange Size Standard; Eurogentec MapMarker 500 |
| GS600 | (40), (60), 80, 100, 114, 120, 140, 160, 180, 200, 214, 220, 240, 250, 260, 280, 300, 314, 320, 340, 360, 380, 400, 414, 420, 440, 460, 480, 500, 514, 520, 540, 560, 580, 600 | Applied Biosystems GeneScan 600 |
| SS600 | (60), 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, 420, 440, 460, 480, 500, 520, 540, 560, 580, 600, 620, 640 | SCIEX DNA Size Standard Kit 600 |
1 Fragments between brackets are recognised but are not used by Coffalyser.Net to determine the sizing curve, e.g. because their length is unstable.
2 Manufacturers may change fragment sizes. Please confirm the sizes if you experience difficulties with size calling.
Less common size markers
| Name | Fragments 1 | Examples of Size Standards 2 |
|---|---|---|
| DY632-500 | 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 250, 260, 280, 290, 300, 320, 340, 360, 380, 400, 420, 440, 460, 480, 500 | - |
| ET400 | (60), (90), 100, 120, 150, 160, 170, 190, 200, 220, 250, 270, 290, 300, 310, 330, 350, 360, 380, 400 | GE Healthcare MegaBACE ET400-R |
| ET550 | (60), (75), 100, 125, 150, 175, 200, 225, 250, 275, 300, 310, 325, 350, 375, 400, 425, 450, 475, 500, 525, (550) | GE Healthcare MegaBACE ET550-R |
| ET900 | (60), (75), 100, 125, 150, 175, 200, 225, 250, 275, 300, 310, 325, 350, 375, 400, 425, 450, 475, 500, 525, 550, 575, 600, 610, 625, 650, 675, 700, 725, 750, 775, 800, 825, 850, 875, (900) | GE Healthcare MegaBACE ET900-R |
| GeneTek-500 | (60), 80, 100, 113, 120, 140, 160, 180, 200, 215, 220, 240, 260, 280, 300, 320, 330, 340, 360, 380, 400, 420, 430, 440, 460, 480, 500 | GENETEK Biopharma GT500 Size Standard |
| GS1000 | (47), (51), (55), 82, 85, 93, 99, 126, 136, 262, 293, 317, 439, 557, 692, 695, 946 | Applied Biosystems GeneScan 1000 |
| GS120 | 15, 20, 25, 35, 50, 62, 80, 110, 120 | Applied Biosystems GeneScan 120 |
| GS350 | (35), (50), 75, 100, 139, 150, 160, 200, 250, 300, 340, 350 | Applied Biosystems GeneScan 350 |
| GS400 | (50), (60), 90, 100, 120, 150, 160, 180, 190, 200, 220, 240, 260, 280, 290, 300, 320, 340, 360, 380, 400 | Applied Biosystems GeneScan 400 |
| GS500-250 Short | (35), (50), (75), 100, 139, 150, 160, 200, (250), 300, 340, 350, 400, 450, 490, 500 | Included for troubleshooting purposes. |
| GT1000 | (50), 75, 100, 125, 150, 200, 250, 300, 350, 400, 450, 475, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000 | - |
| GT500 | (50), 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, 425, 450, 475, 490, 500 | - |
| GT500+ | (35), (50), 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, 500 | - |
| ILS-600 | 60, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 550, 600 | Promega Internal Lane Standard 600 |
| LS500 | 90, 100, 110, 140, 150, 160, 190, 200, 210, 250, 290, 300, 310, 340, 350, 400, 450, 500 | - |
| MM400 | (70), (80), (90), 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400 | Eurogentec MapMarker 400 |
| MM500 | (70), (80), (90), 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, 450, 490, 500 | - |
| MRC550 | (57), 68, 78, 87, (98), 107, 119, 124, 127, 160, 180, 200, 204, 207, 240, (280), 300, 305, 309, 340, (380), 400, 405, 409, 440, (460), (480), 500, 506, 511, 550 | - |
| ROX500 | 50, 70, 90, 110, 130, 150, 170, 190, 200, 210, 230, 270, 290, 300, 310, 330, 350, 370, 390, 410, 430, 450, 470, 490, 500 | - |
| SALSA550 | 70, 80, 90, 100, 120, 124, 127, 160, 200, 240, 300, 305, 309, 340, 400, 440, (470), 500, 506, 511, 550 | - |
| SS400 | (60), 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, 420 | SCIEX DNA Size Standard Kit 400 |
| SS600end | (60), 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, 420, 440, 460, 480, 500, 520, 540, 560, 580, 600 | Included for troubleshooting purposes. |
| SS600Min | (60), 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, 420, 440, 460, 480, 500, 520, 540, 560 | Included for troubleshooting purposes. |
1 Fragments between brackets are recognised but are not used by Coffalyser.Net to determine the sizing curve, e.g. because their length is unstable.
2 Manufacturers may change fragment sizes. Please confirm the sizes if you experience difficulties with size calling.