It is not possible to order custom MLPA probes or probemixes from MRC Holland. However, we are always interested in your suggestions and ideas, and we do continue with some requests.
||The information in this article applies to conventional MLPA, not to digitalMLPA. It is currently not possible to make synthetic digitalMLPA probemixes.|
If you are interested in a gene or region that is not covered by our existing products, we recommend that you contact us first to discuss the possibilities. In some cases, we may already have a restricted product or a product in development that aligns with your interests.
All other suggestions and requests are evaluated by our product development department. In general, we are most likely to develop new probes or probemixes if we think the application is of broad interest. Therefore, it helps if you include the following information in your request:
- Information on the medical condition associated with aberrations in the target area.
- The prevalence of copy number changes and point mutations in the medical condition.
- Any references that may help us evaluate your request.
If we decide to continue with your request, all development costs are paid by MRC Holland. The developed product will generally first be shared with the requesting labs. Once the product has been proven to be useful, it will usually be released to all our customers. In some cases, we can keep the developed probemix exclusive to the requesting lab(s), e.g. until a publication has been accepted. Please ask us about the possibilities.
It is important to note that the development and production of a new product can take a significant amount of time. If we cannot help you or if you cannot wait for us to design a new product, you can also design your own MLPA probemix. This is a common practice performed by many of our customers. We have a protocol available that describes the design of synthetic probes in detail. We also provide two MLPA probemixes with reference probes and control fragments to which your self-designed synthetic probes can be added: P300 Reference-2 (recommended for a smaller number of probes) and P200 Reference-1 (recommended for a large number of synthetic probes). Custom probes cannot be ordered from MRC Holland, but have to be obtained from an oligonucleotide supplier, such as IDT. Please note that the probe design process is relatively complicated, and that we cannot provide additional support on the design of synthetic probes.