SALSA® digital Multiplex Ligation-dependent Probe Amplification (digitalMLPA™) is a multiplex PCR followed by Illumina sequencing-based amplicon quantification, for the detection of copy number variations (CNV) and specific point mutations. digitalMLPA amplifies ligated probes with a universal primer pair enabling unbiased amplification. With digitalMLPA, up to 1000 unique sequences can be detected and quantified in a single reaction.
The digitalMLPA technique is similar to conventional MLPA®, the gold standard for CNV detection of multiple DNA sequences, but with the ability to examine many more targets in a single reaction. digitalMLPA samples can be combined with other NGS sequencing libraries in a single run to give simultaneous results for reliable CNV quantification and NGS sequence analysis. This saves time and money, ensuring sample result turnaround times are met.
This brochure provides an overview of the digitalMLPA technique. More information about our products can be found in one of our application-specific brochures, via our assay finder, or in our full product catalogue.