MLPA requires a capillary electrophoresis device that uses denaturing conditions. Devices that use non-denaturing conditions are not suitable for MLPA. This includes the Agilent (formerly Advanced Analytical/AATI) Fragment Analyzer, the Agilent 2100 Bioanalyzer, the Agilent TapeStation systems, the Caliper LabChip GX/GXII and the QIAGEN QIAxcel.
The use of non-denaturing conditions can alter the relative mobility of the MLPA reaction products. As a result, the distance between some peaks may be larger or smaller than under denaturing conditions. In some cases, fragments may even swap positions in the electropherogram due to secondary structure formation.
At MRC Holland the distance between each fragment is optimized based on denaturing electrophoresis conditions. Therefore, our products are not suitable for use with electrophoresis devices that use non-denaturing conditions.
For a list of supported capillary electrophoresis devices please refer to our current protocol.
The information provided in this material is correct for the majority of our products. However, for certain applications, the instructions for use may differ. In the event of conflicting information, the relevant instructions for use take precedence.