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Is it possible to detect SNVs with (digital)MLPA? - Help Centre / MLPA & Coffalyser.Net / MLPA Technique - MRC Holland Support

Is it possible to detect SNVs with (digital)MLPA?

It is possible to detect known SNVs with MLPA and digitalMLPA, but these techniques are not suitable for the detection of unknown SNVs. Both MLPA and digitalMLPA are primarily used to identify deletions and duplications.

Background

The MLPA and digitalMLPA techniques involve the ligation of two probe oligos that are hybridised to immediately adjacent locations on the sample DNA. This ligation step is highly specific and will be inhibited if there is a mismatch around the ligation site. This allows the design of probes that are specific for either the SNV of interest, or for the reference (wild-type) sequence at the location of a known SNV.

This principle is used to add probes for common SNVs to some (digital)MLPA probemixes. However, this method is not suitable for the detection of a large number of different SNVs, as this would require the design of specific probes for each individual SNV.

Note
Read more about the principle of the MLPA and digitalMLPA techniques.
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The information provided in this material is correct for the majority of our products. For certain applications, the instructions for use may differ. In the event of conflicting information, the relevant instructions for use take precedence.