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MLPA & Coffalyser.Net

MLPA Technique (10)

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• Getting started with MLPA
• What is MLPA, and how does it work?
• Video: How does MLPA work?
• What are the main advantages and limitations of MLPA?
• How does methylation-specific MLPA (MS-MLPA) work?

View 10 articles

Educational Resources (11)

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• Educational resources for MLPA: e-learning, videos, workshops & webinars
• Video: How does MLPA work?
• Video: How to perform an MLPA reaction
• Video: How to analyse SALSA MLPA data in Coffalyser.Net
• Video: How to analyse SALSA MS-MLPA data in Coffalyser.Net

View 11 articles

Experimental Setup (15)

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• Video: How to perform an MLPA reaction
• What are the sample and buffer requirements for (digital)MLPA?
• What control samples should be included in (digital)MLPA experiments?
• List of verified positive samples that can be used with (digital)MLPA probemixes
• Is the purity and quality of the sample DNA important for (digital)MLPA?

View 15 articles

Coffalyser.Net Data Analysis Software (68)

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• Using Coffalyser.Net to analyse MLPA data
• Installation Manual for Coffalyser.Net
• Reference Manual for Coffalyser.Net
• Video: How to analyse SALSA MLPA data in Coffalyser.Net
• How do I adjust a bin set (create a manual bin set) in Coffalyser.Net?

View 68 articles

Raw Data Interpretation & Troubleshooting (9)

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• Troubleshooting MLPA results
• MLPA troubleshooting wizard
• What are the Q- and D-fragments that are present in SALSA MLPA probemixes?
• What are the peaks in the no-DNA control of my MLPA experiment?
• Which factors influence variability of (digital)MLPA probes?

View 9 articles