-
Is it possible to use DNA extracted from formalin-fixed paraffin-embedded (FFPE) tissue for (digital)MLPA?
It is possible to use DNA extracted from FFPE tissue for a limited number of conventional MLPA and d...
-
What are the sample and buffer requirements for (digital)MLPA?
The main sample requirements for conventional MLPA and digitalMLPA are listed below. Note The instr...
-
Which factors influence variability of (digital)MLPA probes?
Variability in conventional MLPA and digitalMLPA experiments can be introduced by several factors, i...
-
Is the presence of low signals in a digitalMLPA no-DNA reaction problematic?
A low number of reads in digitalMLPA no-DNA reactions is usually not problematic. The number of uniq...
-
Ethanol precipitation protocol
DNA precipitation by ethanol can be used to concentrate highly diluted DNA samples and to remove cer...
-
What do the product version numbers mean?
We use version numbers to make sure that changes to a product are noticed by our customers. Our vers...
-
Can multiple digitalMLPA probemixes, versions or experiments be combined in a single run?
Yes, it is possible to combine samples that have been tested with different digitalMLPA probemixes o...
-
Do you have digitalMLPA sample data available?
We have several data demo sets available with sample data of different SALSA® digitalMLPA™ probemixe...
-
Can I assess my digitalMLPA reactions or library using a TapeStation?
The digitalMLPA™ technique has been designed in such a way that library preparation can be done quic...
-
Can digitalMLPA be combined with other NGS libraries?
It is possible to combine digitalMLPA with other sequencing libraries in a single next-generation se...
-
Can the single-end 75 cycle NextSeq High Output Kit v2 be used for digitalMLPA?
The Illumina NextSeq 500/550 High Output Kit (1× 75 cycles; Illumina catalogue nr. 20024906) can be ...
-
Getting started with digitalMLPA
Note This article is about digitalMLPA. For information about conventional MLPA, see this article. ...
-
What control samples should be included in (digital)MLPA experiments?
Every conventional MLPA experiment must include at least three independent DNA reference samples. Fo...