All MLPA probemixes include a set of quality control fragments that are used to aid in the determination of the quality of MLPA data. These include the 92 nt benchmark fragment and the 88 and 96 nt denaturation fragments (D-fragments). It is essential when performing an MLPA analysis that these fragments are correctly recognised (probe signal falls inside a bin). When the quality control fragments are not correctly binned, this can affect Coffalyser.Net’s quality control score calculations, resulting in either an overestimation or underestimation of data quality.
In order to ensure that all your probe signals have been correctly binned a manual bin set should be made. Making a manual bin set is a mandatory first step when performing MLPA analysis in a diagnostic setting.
Read more about the importance of creating a manual bin set in this document.
The information provided in this material is correct for the majority of our products. However, for certain applications, the instructions for use may differ. In the event of conflicting information, the relevant instructions for use take precedence.