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Depurination and MLPA

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Depurination v03 (CSM.EXPS-002).pdf (286.98 KB)

Depurination of sample DNA can affect probe hybridisation and can lead to the reduction or complete failure of a probe to ligate, which can lead to variability. This document explains what depurination is, how it affects MLPA experiments, and how it can be prevented. A summary of the information in this document can be found in this article.


This document contains information specific to conventional MLPA. For information about digitalMLPA, see this article.

Related Pages

  • How do I prevent sample depurination?

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The information provided in this material is correct for the majority of our products. However, for certain applications, the instructions for use may differ. In the event of conflicting information, the relevant instructions for use take precedence.


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