Depurination of sample DNA can affect probe hybridisation and can lead to the reduction or complete failure of a probe to ligate, which can lead to variability. This document explains what depurination is, how it affects MLPA experiments, and how it can be prevented. A summary of the information in this document can be found in this article.
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This document contains information specific to conventional MLPA. For information about digitalMLPA, see this article. |